Related Experiment Video
Updated: Oct 25, 2026
In Vivo Vascular Injury Readouts in Mouse Retina to Promote Reproducibility
Published on: April 21, 2022
Exploratory structure-function associations in retinal vein occlusion-associated macular edema using deep
Johannes Schrittwieser1, Klaudia Birner1, Laetitia Hinterhuber1
1Department of Ophthalmology and Optometry, Medical University of Vienna, Vienna, Austria.
Objective:
To investigate associations between disease-specific OCT biomarkers and pointwise retinal sensitivity (PWS) in retinal vein occlusion (RVO) derived from 2 microperimetry (MP) devices.
Design:
Exploratory, prospective, cross-sectional study.
Participants:
Twenty eyes of 20 patients with macular edema secondary to RVO.
Methods:
Patients underwent spectral-domain OCT (Spectralis; Heidelberg Engineering) and 2 examinations with each MP device (MAIA2, iCare; MP-3, Nidek). Intraretinal fluid (IRF), subretinal fluid (SRF), and ellipsoid-zone thickness (EZT) were segmented using deep-learning-based algorithms; disorganisation of inner retinal layers (DRIL) and intraretinal hemorrhage were annotated manually. Co-registration of OCT volumes and MP grids enabled pointwise structure-function analyses. Mixed-effects models assessed the associations of biomarkers with PWS, pointwise EZT, and fixation stability (FS).
Results:
Mean age was 66 ± 13 years, 13 patients were male, and 11 eyes had branch and 9 central RVO. Greater EZT was associated with greater PWS (+0.14 dB/µm), whereas greater IRF volume (-1.15 dB/nL) and the presence of SRF (-2.35 dB), DRIL (-3.29 dB), and hemorrhage (-9.33 dB) were associated with lower PWS (all P < 0.001). Greater IRF volume (-0.46 µm/nL) and SRF presence (-6.76 µm) were associated with lower pointwise EZT (both P < 0.001).
Conclusions:
IRF, SRF, hemorrhage, DRIL, and EZT showed associations with PWS, whereas IRF and SRF were associated with lower EZT in RVO. MP may improve understanding of structure-function associations in RVO.