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Updated: Oct 25, 2026
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
Published on: March 10, 2017
Genome-wide human T cell screens: barriers and design
Jie Zhong1, Jiajia Wang2, Chen Wang2
1National Key Laboratory of Immunity and Inflammation, Suzhou Institute of Systems Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Suzhou, Jiangsu 215123, China; School of Science, Xi'an Jiaotong-Liverpool University, Suzhou, Jiangsu 215123, China.
Abstract:
Liu et al. enabled genome-wide clustered regularly interspaced short palindromic repeats (CRISPR) screening of primary human T cells in vivo and identified P2RY8 and GNAS through complementary functional readouts. We discuss how context shapes perturbation effects, and how readouts guide prioritization, with implications for T cell engineering in solid tumors.
