Comparative Study of Hypoglycemic Activity of Cr-Gliclazide and Its β‑Cyclodextrin Inclusion Complex
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Updated: Oct 26, 2026

An In Ovo Model for Testing Insulin-mimetic Compounds
Published on: April 23, 2018
Khalid Rasheed1, Christy Munir1,2, Anum Hayat3
1Department of Chemistry, Quaid-e-Azam University, Islamabad 15320, Pakistan.
Abstract:
This study investigated the synthesis and pharmacological properties of chromium metal complexes with sulfonylureas (hypoglycemic agents). In these complexes, the structural element (chromium) is held within the cavity of the β-cyclodextrin (βCD) molecule. Conditions were optimized for the inclusion complex: 1:1 host-guest ratio, pH = 12, and temperature: ∼25 °C. Cr-(III)-Gliclazide (GCZ) and βCD under appropriate experimental conditions show circular dichroism (CD) bands in the visible region: positive Cotton bands at 24,691 cm-1 (Δε = 20.103 degree L mol-1 cm-1) and 16,666 cm-1 (Δε = 26.6 × 10-3 degree L mol-1 cm-1) and a strong negative Cotton band at 19,230 cm-1 (Aε = 38.1 × 10-3 degree L mol-1 cm-1). The synthesized complexes were assessed for their hypoglycemic activity in rabbits with alloxan-induced diabetes. Cr-(III)-GCZ showed an early therapeutic response that persisted over an extended duration, while the Cr-(III)-GCZ-βCD inclusion complex showed a delayed therapeutic response but maintained an extended duration of activity compared with the reference (parent) drug, gliclazide (GCZ). The synthesized complexes exhibited good therapeutic potential, and the toxicity of these complexes was found to be quite low (high LD50 values).
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