Crystal structure of tyrosine-tRNA synthetase from Helicobacter pylori G27
David Igbalajobi1, Jason E Taylor1, Raymond Gao2
1Dartmouth College, Hanover, New Hampshire, USA.
Abstract:
Helicobacter pylori, a major cause of gastric ulcers and cancers, infects nearly half of the world's population. The crystal structure of a potential H. pylori structure-guided drug-discovery target, tyrosine-tRNA synthetase (HpTyrRS), has been determined by the Seattle Structural Genomics Center for Infectious Disease (SSGCID). HpTyrRS is an enzyme that catalyzes the ATP-dependent attachment of tyrosine to tRNA. HpTyrRS was produced and crystallized, and its structure was determined at 2.2 Å resolution. HpTyrRS has a prototypical bacterial TyrRS topology and forms dimers in solution. HpTyrRS shares a conserved binding cavity for a previously identified Staphylococcus aureus TyrRS inhibitor (SB-219383). The reported structure and analysis establish HpTyrRS as a potential target for structure-guided drug discovery and repurposing.
More Related Videos
Related Concept Videos
ATP Synthase: Structure
RNA Structure
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
RNA Structure
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
tRNA Activation
Transfer RNA Synthesis
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
Protein and Protein Structure


