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Updated: Oct 26, 2026
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
Published on: April 13, 2015
Single-cell analysis reveals the potential immunoregulatory role of C3 + malignant cell subpopulations in
Shengyun Hu1,2, Yatong Dang1,2, Baojing Tuo1,2
1Department of Colorectal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052, China.
Background:
Dedifferentiated liposarcoma (DDLPS), a rare disease with limited research, exhibits resistance to radiotherapy and chemotherapy. Few reports have analyzed the tumor microenvironment of retroperitoneal undifferentiated liposarcoma using single-cell sequencing.
Methods:
Single-cell analysis of three retroperitoneal DDLPS cases characterized the cellular and immune microenvironment.
Results:
Our findings reveal significant heterogeneity among malignant cells in DDLPS, with chromosome 6 deletions being prevalent across malignant cell subpopulations and potentially linked to reduced immunogenicity based on pathway analyses. Notably, the coexistence of CD8 + T cell exhaustion and cytotoxicity reflects a continuum of exhaustion transition. Moreover, we identify a C3 + malignant cell subpopulation with ECM remodeling, angiogenesis, and activated complement pathway. Further exploration suggested that C3 + malignant cells may recruit IL2RA+ regulatory T cells and SPP1 + macrophages through the C3-C3aR1 axis.
Conclusion:
Our findings provide new insights into the application of immunotherapy in sarcoma.
