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Updated: Oct 26, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
Published on: January 13, 2017
Polyketides from a soil -derived Streptomyces sp. CSDX076 and their antimicrobial activities
Shengyan Qian1,2, Hengxing Cai3, Afsar Khan4
1Guizhou Provincial Key Laboratory of Microbial Resources and Drug Development, Zunyi Medical University, Zunyi, Guizhou, China.
Abstract:
A new naturally occurring polyketide, pseurotin A3-10,11-acetonide (1), along with two known analogs, pseurotin A (2) and pseurotin A3 (3), was isolated from the culture broth of Streptomyces sp. CSDX076. The structures of these compounds were determined by extensive 1D and 2D nuclear magnetic resonance (NMR), ultraviolet (UV), and high-resolution electrospray ionization mass spectrometry (HRESIMS) spectroscopic analyses. All isolated compounds (1-3) were evaluated for antimicrobial activity against a panel of seven pathogenic strains using disc diffusion and broth microdilution methods. Notably, compound 1 exhibited moderate activity against Brucella melitensis with a minimum inhibitory concentration (MIC) of 8.0 μg/mL, while compound 2 showed weak activity against Staphylococcus epidermidis (MIC = 128.0 μg/mL); compound 3 was inactive against all tested strains. This study reports, for the first time, the antibacterial effects of these compounds against the respective pathogens.
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