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From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia
Published on: October 19, 2014
Expression of TCTP/HRF and MCL-1 in chronic lymphocytic leukemia: implications for disease pathogenesis
Handan Kayhan1, Asuman Sunguroglu2, Sanem Gokcen3
1Faculty of Medicine, Department of Adult Hematology, Gazi University, ANKARA, Emniyet Mahallesi, Mevlana Bulvarı, No:29, Yenimahalle, 06560, Turkey.
Background:
Chronic lymphocytic leukemia (CLL) is characterized by defective apoptosis and overexpression of anti-apoptotic proteins. Translationally Controlled Tumor Protein/Histamine-Releasing Factor (TCTP/HRF) is a highly conserved multifunctional protein with anti-apoptotic properties that stabilizes myeloid leukemia cell differentiation protein (Mcl-1).
Objective:
To investigate TCTP and Mcl-1 expression levels in CLL patients compared with healthy controls and examine their clinical correlations.
Methods:
We analyzed mRNA and protein levels of TCTP using Quantitative PCR (qPCR) and flow cytometry in 54 CLL patients and 22 healthy controls. Clinical parameters, prognostic markers, and cytogenetic abnormalities were correlated with expression levels.
Results:
Both TCTP and Mcl-1 mRNA and protein levels were significantly elevated in CLL patients compared with controls. TCTP and Mcl-1 protein expression levels showed a strong positive correlation. TCTP mRNA, Mcl-1 mRNA, TCTP protein, and Mcl-1 protein expression levels were positively correlated with lymphocyte counts in the evaluable CLL cohort, whereas TCTP mRNA, TCTP protein, and Mcl-1 protein expression levels were inversely correlated with platelet counts in newly diagnosed patients. TCTP and Mcl-1 mRNA expression levels were significantly lower in ZAP-70-positive patients. No significant associations were observed with disease stage, cytogenetic abnormalities, or CD38 expression.
Conclusions:
These findings suggest a potential role for the TCTP/Mcl-1 axis in CLL pathogenesis and progression, warranting further investigation as therapeutic targets.
