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Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
Published on: August 18, 2018
Erebosis: An Emerging Concept of a New Type of Regulated Cell Death
Sergey V Nikulin1,2, Dmitry M Hushpulian1, Arpenik A Zakharyants1,3
1HSE University, Moscow, Russia.
Abstract:
This review briefly describes the discovery of a morphologically distinct form of regulated cell death in Drosophila, termed erebosis, which is characterized by organelle loss, nuclear flattening, and intracellular accumulation of Ance. The latter serves as a biomarker of this type of cell death but does not act as its trigger. Erebosis can be induced by amino acid deprivation accompanied by the reduced cytoplasmic viscosity, and its development is also associated with the increased heme oxygenase activity and decreased intracellular heme levels. We hypothesize that the heme-sensing protein E75 in Drosophila melanogaster, a homolog of mammalian REV-ERB proteins involved in circadian regulation, may provide a link between erebosis and renewal of enterocytes from the intestinal stem cells. Finally, we discuss the differences between erebosis and other forms of regulated cell death, as well as detection of ACE2 in the brains of mice in different models of Alzheimer's disease.
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