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Updated: Oct 28, 2026
Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
Published on: February 15, 2021
Modeling Skeletal Muscle Stem Cell Fate In Vitro
Muriel Sébastien1, Emilie Christin1, Vincent Gache2
1Pathophysiology and Genetics of Neuron and Muscle (PGNM), NeuroMyoGene Institute, CNRS UMR5261, Inserm U1315, Université Claude Bernard Lyon 1, Lyon, France.
Abstract:
Skeletal muscle stem cells (MuSCs) are necessary for muscle development and regeneration. Upon external cues, they activate from their quiescent state to self-renew and/or differentiate. The study of MuSCs is challenging as they tend to differentiate when isolated from their microenvironment. Here, we describe an in vitro model that mimics a skeletal muscle environment, allowing MuSC preservation together with myofiber maturation.
