Autonomic dysregulation and high arterial blood pressure in male Crtc1-deficient mice

Epidemiological evidence links cardiovascular disease, sympathetic nervous system overactivity, obesity, and depression. Whether this association is causal and what mechanisms may be involved remains unknown. Mice deficient in the cAMP response element-binding protein-regulated transcription coactivator 1 (CRTC1) show depression-related behavior and a metabolic profile resembling the metabolic syndrome. This study aimed to characterize arterial blood pressure regulation in these mice to determine whether global CRTC1 deficiency is also associated with arterial hypertension, another key characteristic of the metabolic syndrome. Telemetric measurements in unrestrained male mice revealed high blood pressure in Crtc1-deficient animals before the onset of obesity or hypertrophy of arterioles, accompanied by decreased heart rate variability, increased systolic blood pressure variability, enhanced environmental stress sensitivity and increased urinary catecholamine excretion. Pharmacological α₁-adrenoceptor blockade with prazosin induced greater blood pressure reductions in Crtc1-deficient mice and eliminated genotype-dependent pressure differences, whereas the β₁-adrenoceptor antagonist metoprolol showed no genotype-specific effect. Regulation of renal sodium transport proteins, activity of the renin-angiotensin-aldosterone system, and cardiac systolic function were unchanged between genotypes. Crtc1-deficient animals also exhibited impaired glucose tolerance and a depression-related phenotype associated with reduced locomotion, increased agmatinase, and decreased cerebral brain-derived neurotrophic factor (Bdnf) and c-fos expression. These findings indicate that CRTC1 deficiency leads to high blood pressure, likely driven by excessive peripheral α₁-adrenoceptor-mediated vasoconstriction caused by autonomic dysregulation. The co-occurrence of increased blood pressure, metabolic dysregulation, and the depressive-like phenotype in male Crtc1-deficient mice suggests a regulatory role of CRTC1 in the complex central network of signaling pathways involved in the frequent comorbidity of metabolic syndrome and depression.NEW & NOTEWORTHY Male Crtc1-deficient mice develop autonomic dysfunction and high arterial blood pressure, likely driven by enhanced α₁-adrenergic vasoconstriction and independent of obesity or increased arteriolar wall thickness. The co-occurrence of elevated sympathetic activity, early cardiac remodeling, impaired glucose tolerance, and depression-related behavior suggests an involvement of CRTC1-dependent signaling in the coordination of autonomic, metabolic, and affective phenotypes.