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Updated: Oct 28, 2026

Genome Editing in Primary Mammalian Cells via Electroporation of Editor RNA
Published on: July 31, 2026
Efficient and precise large-scale genomic duplication using amplification editing
Si-Si Xie1,2, Zhou He1,2, Hao Yin3,4,5
1State Key Laboratory of Virology and Biosafety, Departments of Clinical Laboratory, Neurosurgery and Urology, Medical Research Institute, Frontier Science Center for Immunology and Metabolism, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, China.
Abstract:
Genomic duplication is a common type of structural variation that has important roles in gene dosage regulation and contributes to the development of various human diseases including cancer and inherited disorders. However, current genome-editing technologies are limited in their ability to introduce precise and efficient DNA duplications. Amplification editing (AE) is a genome-editing strategy that enables efficient and precise duplication of genomic sequences ranging from tens of base pairs to chromosomal scales. AE offers high precision and efficiency and the ability to duplicate endogenous sequences across multiple targeting sites and in diverse cell types. Here, we describe detailed procedures for the design, execution and analysis of AE-based genomic duplication. These include protocols for duplicating endogenous loci across a range of fragment sizes, as well as workflows to quantify editing efficiency and validate duplication outcomes. In addition, we provide guidance for optimizing AE performance and extending its applicability across diverse genomic targets and experimental contexts. Most AE experiments can be completed within 2 weeks, supporting efficient implementation of targeted genomic duplication in mammalian cells.
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