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miR-31 Expression Correlates with Radiation-Induced Bystander Responses in Breast Cancer Cells
Seyedeh Simin Mosavi Avendi1, Rahil Ghanbarnasab-Behbahani1, Amir Danyaei1,2
1Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Purpose:
This study investigated the association of miR-31 expression with radiation-induced bystander responses in non-irradiated MDA-MB-231 cells exposed to radiation-conditioned medium derived from irradiated MCF-7 breast cancer cells.
Methods:
MCF-7 cells were exposed to 0.5, 1, 2, and 4 Gy of 6 MV X-rays. Conditioned media were collected at 0, 24, and 48 hours' post-irradiation and applied to MDA-MB-231 cells. Cell viability was assessed via MTT assay. Apoptosis and cell cycle behaviors were assessed using flow cytometry. Wound healing assays were conducted to assess migration, and RT-qPCR was used to quantify miR-31 expression.
Results:
MDA-MB-231 cells underwent dose- and time-dependent alterations associated with conditioned media from irradiated MCF-7 cells. Apoptosis increased significantly over time, especially in the 2 and 4 Gy groups. Cell cycle analysis showed shifts in the G1 and S phases. Migration rates were enhanced at specific time points, particularly in the 0.5 and 2 Gy groups. The expression of miR-31 was significantly increased in treated MDA-MB-231 cells.
Conclusion:
Our findings suggest a potential association between altered miR-31 expression and radiation-induced bystander responses in breast cancer cells. Further mechanistic studies are required to clarify its functional role and therapeutic relevance.