Related Experiment Video
Updated: Oct 28, 2026
Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry
Published on: May 5, 2017
Addressing complex autofluorescence signatures in solid tissue samples to enhance full spectrum flow cytometry of
Christina Gkantsinikoudi1, Manuela Terranova-Barberio2, Neil Dufton1
1Centre for Microvascular Research, William Harvey Research Institute, Queen Mary University of London, EC1M 6BQ, United Kingdom.
Abstract:
FSFC is an emerging technology that can greatly enhance our understanding of the single-cell proteomic landscape. We demonstrate that this application is applicable to cells derived from solid tissues which have traditionally been hampered by their complex autofluorescence signatures and lack of optimized tools for non-immune cells. We present a protocol and discuss key controls that minimize the impact of unmixing errors, enabling us to resolve multiple EC subpopulations isolated from different tissues in models of chronic tissue injury.
Research Topics:
vascular biology, cell heterogeneity, full spectrum flow cytometry.
