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Updated: Oct 28, 2026

Methodology for Sputum Induction and Laboratory Processing
13:28

Methodology for Sputum Induction and Laboratory Processing

Published on: December 17, 2017

Comment on: A novel method to assess airway eosinophilia using sputum plugs

Einat Fireman-Klein1,2, Elizabeth Fireman3

  • 1Pulmonary Division, Carmel Medical Center, Haifa, Israel.

ERJ Open Research
|October 27, 2026
PubMed

Abstract:

A simplified method to detect eosinophils from induced sputum https://bit.ly/4eD4Ik2.

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Methodology for Sputum Induction and Laboratory Processing
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Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture01:26

Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture

Sputum studies are a critical part of diagnosing and treating numerous respiratory conditions. These studies involve obtaining sputum samples for analysis to identify pathogenic organisms and assess the presence of abnormal cells indicative of malignant conditions. This lesson will delve into three fundamental sputum studies: Gram Stain, Cytology, and Acid-fast Smear and Culture.
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
Sputum Studies II: Culture and Sensitivity01:20

Sputum Studies II: Culture and Sensitivity

Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...

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