Proteomic and Metabolomic Analyses of Lung Squamous Cell Carcinoma

Background/Objectives: As the leading cause of global cancer mortality, lung cancer suffers from low survival rates and an incomplete understanding of its pathogenesis. Lung squamous cell carcinoma (LSCC) involves dynamic protein and metabolic dysregulation. To gain initial molecular insights into LSCC is therefore essential to explore coordinated molecular alterations and early mechanisms in primary LSCC tissues. Methods: In this pilot study, we integrated proteomic and non-targeted metabolomic profiling to analyze six paired human LSCC tissue specimens and matched adjacent non-cancerous tissues. Results: Preliminary analyses revealed extensive differences at both the proteomic and metabolomic levels. Specifically, proteomic profiling highlighted widespread dysregulation in cell adhesion dynamics, whereas non-targeted metabolomic profiling identified perturbations in several amino acid metabolic pathways. Integrated multi-omics analysis suggested potential co-variation patterns linking endoplasmic reticulum (ER) protein processing, cell adhesion, and amino acid metabolism. Conclusions: Collectively, these pilot findings provide an initial exploratory characterization of paired proteomic and non-targeted metabolomic alterations in LSCC. Given the small cohort size, these results serve as hypothesis-generating molecular signatures, and all findings require confirmation in larger, independent cohorts with orthogonal and functional validation.