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Hubei virga-like virus 2 in Aedes aegypti: Identification of a conserved 216-bp sequence missing from the RefSeq
Laura E Guerrero-Martínez1, Samantha Solís-Vidal1,2, Martha Yocupicio-Monroy1
1Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, Mexico City, México.
Abstract:
We report two near-complete genome sequences of Hubei virga-like virus 2 (HVLV-2) from Aedes aegypti in Oaxaca, Mexico. Both genomes contain a 216-bp insertion region absent from the reference sequence but present in all other reported isolates. Split-read analysis and de novo assembly of our samples, along with a reanalysis of raw reads from the reference sequencing project, confirm that this region is present in the original genetic material from which the assemblies were built. Altogether, these findings confirm that A. aegypti is a putative host of HVLV-2 and demonstrate that the insertion is conserved but missing from the RefSeq standard.
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