Related Experiment Video
Updated: Oct 29, 2026

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
Published on: March 2, 2014
Reciprocal regulation between NDP52 and PRRSV: a bidirectional host-virus interplay
Heyou Yi1, Shaojun Wang2, Guijie Guo1
1Key Laboratory of Animal Pathogen Infection and Immunology of Fujian Province, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Joint Laboratory of Animal Pathogen Prevention and Control of Fujian-Nepal, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Abstract:
Porcine reproductive and respiratory syndrome virus (PRRSV) is a major swine pathogen responsible for substantial economic losses worldwide. The selective autophagy receptor NDP52 plays a critical role in antiviral defense, yet its function during PRRSV infection remains poorly understood. In this study, we show that NDP52 restricts PRRSV replication: overexpression reduced viral titers and N protein levels, while knockdown enhanced replication. NDP52 specifically reduced Nsp12 protein levels, which was partially reversed by the autophagy inhibitor 3-methyladenine (3-MA). Conversely, PRRSV infection decreased NDP52 protein levels at late stages without affecting its mRNA levels. Screening of viral proteins identified Nsp1α, Nsp10, and GP3 as being associated with reduced NDP52 levels; this reduction was blocked by 3-MA. Together, these findings suggest a bidirectional host-virus interplay, in which NDP52 restricts viral replication by reducing Nsp12, while the virus counteracts this restriction via Nsp1α, Nsp10, and GP3.The precise mechanisms, including the role of autophagy, require further investigation.
Related Concept Videos
Respiratory Syncytial Virus Disease
Viruses with RNA Genomes
Retrovirus Life Cycles
Viral Recombination
Inhibitors Of Virion Release
Inhibitors of Viral Protein Synthesis

