Related Experiment Video
Updated: Oct 29, 2026

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
Published on: July 14, 2015
rustybam: a composable toolkit for alignment analysis and visualization with SafFire
Cade Mirchandani1, Mitchell R Vollger1
1Department of Human Genetics and Utah Center for Genetic Discovery, University of Utah, Salt Lake City, UT, USA.
Summary:
We present rustybam, a Rust-based command-line toolkit for CIGAR-aware manipulation of PAF and BAM alignments, and SafFire, a browser-based tool for interactive genome comparison visualization with annotation overlays. rustybam's composable subcommands provide overlap resolution, coordinate liftover, alignment splitting at structural variants, scaffolding, and identity statistics.
Availability And Implementation:
Both tools are open source under the MIT license. rustybam is available via Bioconda (https://anaconda.org/bioconda/rustybam), crates.io, and GitHub (https://github.com/vollgerlab/rustybam). SafFire is hosted at https://vollgerlab.com/SafFire/ with source code at https://github.com/vollgerlab/SafFire. Archived versions are available on Zenodo: rustybam (https://doi.org/10.5281/zenodo.4654875) and SafFire (https://doi.org/10.5281/zenodo.5765964).
Supplementary Information:
Supplementary data are available at Bioinformatics online.
Related Concept Videos
Relative Motion Analysis using Rotating Axes
However, to express the relative position of point B relative to point A, an additional frame of reference, denoted as x'y', is necessary. This additional frame not only translates but also rotates relative to the fixed frame, making it instrumental in...
Atomic Orbitals
Relative Motion Analysis using Rotating Axes-Problem Solving
Here, in order to determine the magnitude of velocity and acceleration for point...
Stability of structures
Mesh Analysis
A fundamental concept in mesh analysis is the definition of meshes and mesh currents. A mesh is a closed...
Relative Motion Analysis using Rotating Axes - Acceleration
Time differentiation is...