Integrative characterization of cell-surface proximity interactions using DTSSP crosslinking and high-dimensional profiling
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Published on: June 23, 2013
Myint Myat Thu1, Nuchjira Takheaw1, Witida Laopajon1
1Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand; Biomedical Technology Research Center, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at the Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand.
Abstract:
Extracellular protein interactions mediated by cell surface receptors are fundamental to intercellular communication and immune regulation. Identifying novel ligand-receptor interactions helps uncover previously unknown signalling pathways and improves understanding of immune mechanisms. In this study, we introduce a methodology aimed at facilitating the detection of cell-surface binding or proximity through flow cytometry. We demonstrate the efficacy of employing DTSSP (3,3'-dithiobis sulfosuccinimidylpropionate) crosslinking in conjunction with high-dimensional flow cytometry to achieve enhanced detection of cell-surface association while preserving the integrity of single-cell immune profiling. This strategy provides a versatile methodological framework for studying cell-surface interactions across heterogeneous immune cell populations.
