A KIT-6 mesoporous silica nanoparticle-based chemiluminescent aptasensor for sensitive detection of 8-Oxo-dG
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Published on: June 23, 2016
Maryam Imani1, Zahra Vaezi2, Hossein Naderi-Manesh1
1Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University, Tehran, 14115-154, Iran.
Background:
Excessive accumulation of reactive oxygen species disrupts repair mechanisms and causes irreversible damage to lipids, proteins, and DNA. A target-responsive chemiluminescence sensor was developed for the sensitive detection of 8-OxodG, an important biomarker of oxidative DNA damage.
Results:
In this chemiluminescence sensor, acridinium ester (AE) was loaded into KIT-6 mesoporous silica nanoparticles and capping the pores with an 8-oxodG-specific aptamer. In the presence of 8-oxodG, target-aptamer recognition induced aptamer detachment and AE release. The released AE reacted with H2O2 to generate a concentration-dependent chemiluminescence signal. Under optimized conditions, the sensor exhibited a response range of 0.01-100 μM and a detection limit of 4.8 nM in PBS, by achieving a satisfactory recovery rate of 70.4-108.9% with RSD of 12.7-20.0%. The sensor also showed good selectivity and was successfully evaluated in spiked artificial urine with detection limit of 22.03 nM.
Significance:
The combination of aptamer-controlled molecular gating, the interconnected pore structure of KIT-6, and chemiluminescent signal generation provides a sensitive controlled-release platform for 8-oxodG detection and supports its further development for biological-sample analysis.
