An evaluation of blood components storage duration toward determining the reference intervals of coagulopathic metabolic analytes - A cross-sectional analytical study
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Sankalp Sharma1, Suprava Patel2, Mili Patel1
1Department of Transfusion Medicine and Blood Bank, AIIMS Raipur, India.
Introduction:
Biochemical or metabolomic markers of blood storage lesions must be valid and reproducible.
Objectives Of The Present Study:
The objective of this study was to define reference intervals (RIs), decision limits of coagulopathic biochemical analytes in packed red blood cell (PRBC), and random donor platelet (RDP) blood components for predefined storage interval. The study aimed to analyze the storage duration of PRBC and RDP components toward their respective coagulopathic potential.
Materials And Methods:
This was a cross-sectional analytical study in a hospital-based blood center. Eligible blood donors (2020-2022) as reference sample group, stored non-leukodepleted PRBC, and RDP blood units as reference samples. We analyzed PRBC by RGSH, quantitative RBC lactate dehydrogenase (RLDH), and Rhs-CRP. RDP units, PGSH; PCXCL4/PF4; Phs-CRP; platelet fibrin degradation products (PFDPs), and platelet homocysteine. PRBC stored at 2°C-6°C (≤15 days and >15 days) and RDP: 24°C ±2°C (≤3 days and >3 days) for storage changes (Kruskal-Wallis test and two sample t-test) (P ≤ 0.05). Storage changes, if nonsignificant, determine RIs, 95% confidence intervals (95% CIs) of reference distribution, PRBC (42 days), and RDP (5 days). If significant, separate Reference distribution (95% CI) are decision limits for each storage interval. An association of laboratory parameters and storage duration by regression (co-efficient) fit (R-sq.%, P ≤ 0.05).
Results And Conclusions:
We obtained decision limits and stored PRBC having significant differences in median (≤15 days and >15 days) for Rhs-CRP (n= 23 and 21), RLDH (n= 28 and 29) (P= 0.012; <0.0001); (R-sq.% 14.74; P= 0.032; R-sq.% 60.78, P = <0.0001). In RDP units (≤3 days and >3 days), PFDP levels (n = 25 and n= 19) (P< 0.0001); (R-sq.% 28.29, P< 0.0001). We derived RI for RGSH (n= 58) (P= 0.738) in PRBC (42 days) and PCXCL4/PF4 (n= 58) (P= 0.969), homocysteine (n= 27) (P= 0.123); Phs-CRP (n= 58) (P= 0.181) and PGSH (n= 56) (P= 0.511) in RDP units (5 days). RLDH and PFDP indicated the coagulopathic potential of PRBC and RDP blood components, respectively.
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