Metal-organic framework stationary phases for multi-mode liquid chromatography
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Updated: Oct 31, 2026

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
Published on: July 14, 2015
Xing-Feng Hu1, Jian-Ping Zhu1, Xiao-Wen Zhang1
1Jiangsu Key Laboratory of Micro-Nano Sensing and Separation Science for Analytical Chemistry, Jiangsu Key Laboratory of Biofunctional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of New Power Batteries, College of Chemistry and Materials Science, State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Microbial Technology, Nanjing Normal University, Nanjing, China.
Abstract:
Multi-mode liquid chromatography integrates multiple interaction mechanisms, enabling the efficient separation of analytes with diverse polarities and chemical properties in complex samples. Metal-organic frameworks (MOFs) have emerged as highly attractive stationary phases for multi-mode chromatographic separations because their highly designable pore architectures, periodically arranged metal nodes, and tailorable organic ligands enable the incorporation of multiple recognition sites. This review summarizes recent advances in MOF-based mixed-mode liquid chromatographic stationary phases from the perspectives of synthesis methods, separation mechanisms, and chromatographic applications. The synthesis strategies are first reviewed and classified into four types, including pure-MOF, coating-type, embedding-type, and bonding-type stationary phases. The separation mechanisms and chromatographic applications are subsequently discussed according to the separation mode, including RPLC/HILIC dual-mode, RPLC/HILIC/IEC multi-mode, and chiral/NPLC/RPLC/HILIC/IEC multi-mode separations. Finally, the current challenges and future perspectives are discussed to provide guidance for the rational design of high-performance MOF-based mixed-mode stationary phases.
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