Establishment of four induced pluripotent stem cell lines with high polygenic risk score for Parkinson's disease
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Updated: Oct 31, 2026

Automated Production of Human Induced Pluripotent Stem Cell-Derived Cortical and Dopaminergic Neurons with Integrated Live-Cell Monitoring
Published on: August 6, 2020
Maja Bennin Brataas1, Hege Brincker Fjerdingstad2, Helena Homme Lading2
1Department of Neurology, Oslo University Hospital, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway.
Abstract:
Sporadic Parkinson's disease (PD) has an estimated heritability of ∼ 25%. Dermal fibroblasts from four early-onset sporadic PD patients were selected based on high PD polygenic risk scores (PRS) and reprogrammed into induced pluripotent stem cells (iPSCs) using Sendai virus. Lysosomal and microglial open chromatin PRSs were calculated. The iPSC lines exhibited normal karyotypes and STR profiles identical to the donor blood samples, expressed pluripotency-associated markers, and differentiated into derivatives of all three germ layers in vitro. These iPSC lines represent a resource for modelling polygenic, high-risk sporadic PD with a specific focus on lysosomal and microglial genetic contributions.
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