KinomeX: A Source-Linked, Evidence-Traceable Atlas of the Human Kinome

Protein kinase data originate from sources with different identifiers, definitions, and evidence models. KinomeX (https://kinomex.dokhlab.org) is an online platform that aligns the human protein kinase list from KinHub with reviewed entries from UniProtKB/Swiss-Prot and links each record to source-specific evidence. The catalog includes 678 protein entries: 522 KinHub core entries with 536 kinase-domain rows, and 156 reviewed UniProt entries labeled with the Kinase keyword (KW-0418), mostly phosphorylating lipids, nucleotides, sugars, or small molecules. Kinase profiles integrate reviewed annotations, 10,043 experimental structures, AlphaFold models, 1,021,421 ligand bioactivity records, 36,396 tissue-expression data from GTEx, 63,649 ClinVar variants, and live STRING connections. Missing records are marked as unavailable. We developed the Pharmaceutical Development Interest Score (PDIS), which combines citation, clinical-trial, structural, and chemical data with adjustable component weights. These rankings are consistent across numerous weightings (median Spearman ρ = 0.955) and better reflect Pharos target development levels than citation counts alone. Examples highlight 93 under-cited but structurally and chemically accessible kinases and 47 CNS-specific kinases, with evidence on structure, chemistry, and variants. KinomeX enables evidence review and hypothesis formation, using PDIS to indicate documented development efforts rather than biological significance.