Mogroside V regulates autophagy through AMPK/mTOR pathway to ameliorate cigarette smoke-induced acute lung injury
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Published on: March 3, 2023
Wencai Wang1, Wenhao Wang2, Xingchen Liu3
1Department of Cardiothoracic Surgery, Yancheng Third People's Hospital, The affiliated hospital of Jiangsu Vocational College of Medicine, Yancheng, 224000, Jiangsu, China.
Background:
Cigarette smoke causes acute lung injury (ALI). Mogroside V (MV) has been recently found to relieve cough and asthma symptoms and to eliminate phlegm. We tested the potential and mechanism of action of MV in alleviating cigarette smoke-induced ALI.
Methods:
We explored the effects and mechanisms of action of MV by using cigarette smoke extracts (CSE) in lung‑on‑a‑chip and mouse models. The levels of IL-18, IL-1β and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA) and qRT-PCR. Pulmonary gas-blood barrier was detected by qRT-PCR and western blot. TEER and FITC-dextran permeability assay measured cell barrier function. Western blot and qRT-PCR were used to detect the expression of autophagy-related genes and AMPK/mTOR signaling pathway-related proteins.
Results:
CSE treatment caused an increase in inflammatory factors in a lung‑on‑a‑chip model. After MV treatment, the expression of pro-inflammatory factors decreased, the pulmonary gas-blood barrier disruption was improved in response to CSE. MV promoted autophagy and regulated the AMPK/mTOR pathway.
Conclusion:
MV promotes autophagy and ameliorates cigarette smoke-induced ALI through the AMPK/mTOR signaling pathway in vitro and in vivo.
