Regulatory effect of baicalein on intestinal ribosome biogenesis in mice with asthma
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L U Yao1, Wei Lini1,2, Wang Ruiqi1
1Institute of Clinical Basic Medicine of Traditional Chinese Medicine, Chinese Academy of Traditional Chinese Medicine, Beijing 100010, China.
Objective:
To investigate the therapeutic effects and mechanisms of baicalein (BAI) on asthma based on the gut-lung axis theory.
Methods:
This study utilized BALB/C mice, four-dimensional independent data acquisition proteomics, molecular docking, molecular dynamics simulation, and immunohistochemistry to explore how BAI affects ribosome biogenesis in the gut of asthmatic mice.
Results:
We investigated the regulatory effects of BAI on intestinal proteins, and we identified 93 differentially expressed proteins (DEPs) that overlapped among the control group, model group, and BAI (H) group. These proteins were predominantly involved in translation, cytosolic ribosomes, and structural constituents of ribosomes. BAI treatment significantly regulated the ribosome pathway. Eight ribosome biogenesis-related proteins [small ribosomal subunit protein uS9m (MRPS9), ribosomal protein L28 (RPL28), ribosomal protein L32 (RPL32), ribosomal protein S15A (RPS15A), ribosomal protein S23 (RPS23), RNA polymerase I and III subunit C (POLR1C), ribosomal protein L23A (RPL23A), and ribosomal protein S25 (RPS25)] were significantly correlated with pulmonary inflammatory factors. Molecular docking confirmed that RPS25, RPL23A, and POLR1C were primary targets of BAI. Molecular dynamics simulations revealed that the RPL23A-BAI complex exhibited greater structural stability than the RPS25-BAI and POLR1C-BAI complexes. Immunohistochemistry further verified that BAI suppressed the protein expression of RPS25, RPL23A, and POLR1C in the colon.
Conclusions:
These results underscore that the mechanism by which BAI alleviates asthma may involve the regulation of ribosome biogenesis in colonic tissue.
