Protocol for low-input whole-genome bisulfite sequencing in Arabidopsis
Related Experiment Video
Updated: Nov 1, 2026
Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm
Published on: January 28, 2011
Jie Yang1, Binh Thanh Vo1, Frank Johannes1
1Plant Epigenomics, TUM School of Life Sciences, Technical University of Munich, 85354 Freising, Germany.
Abstract:
DNA methylation profiling of rare plant samples is limited by the scarcity of input DNA. Here, we present a protocol for low-input whole-genome bisulfite sequencing (WGBS) in Arabidopsis in which DNA inputs as low as 50 pg can generate methylomes comparable to conventional WGBS. We describe steps for tissue cryomilling, Proteinase K lysis, and direct bisulfite conversion. We then detail procedures for post-bisulfite adapter tagging library construction and MethylStar-based alignment and methylation calling.
