Vitrification of Culter alburnus embryos by laser-assisted warming with gold nanorods
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Updated: Nov 2, 2026
Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
Published on: March 2, 2017
Xinqing Cai1, Yuting Luo1, Hongkuan Song1
1Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; Marine Biomedical Science and Technology Innovation Platform of Lin-gang Special Area, Shanghai, China.
Abstract:
Cryopreservation of fish embryos is highly challenging, and to date, successful reports of fish embryo cryopreservation remain scarce. In this study, we optimized the vitrification solution and employed gold nanorods (GNRs) in a laser-assisted warming process to improve the hatching rate of Culter alburnus embryos after cryopreservation. With V11 vitrification solution (15% DMSO, 10% EG, 15% PG, and 1 mM MT) and a laser-assisted warming condition (25 μg/mL GNRs with continuous 15 W laser irradiation), we achieved rapid and homogeneous warming, and the mean hatching rate of cryopreserved embryos reached 18.83%. RNA-seq revealed that the above cryopreservation procedure led to significant transcriptional changes in calcium signaling pathways and apoptosis-related genes, which may facilitate further optimization of the vitrification solution. This study provides a basis for further improving the efficiency of embryo cryopreservation in Culter alburnus and other fish species.
