Colorimetric chemiluminescent bifunctional sensor for sensitive detection of choline in milk samples
Related Experiment Video
Updated: Nov 6, 2026

Bacterial Detection & Identification Using Electrochemical Sensors
Published on: April 23, 2013
Yongning Song1, Jingkang Li1, Yunmei Wu1
1College of Chemistry, Jilin Province Research Center for Engineering and Technology of Spectral Analytical Instruments, Jilin University, Qianjin Street 2699, Changchun, 130012, China.
Abstract:
Rapid sensors for choline detection in food samples are highly desired. Three quinoline-based sensors (KL-1/2/3) were synthesized, among which KL-1 with favorable chemiluminescence performance was selected. For the first time, quinoline skeletons were utilized to construct TMB-free dual-mode colorimetric-chemiluminescence sensors. Choline oxidase produces H₂O₂ from choline, triggering simultaneous luminescence and color fading on KL-1. This sensor quantifies choline linearly from 10 to 100 μM with LOD of 6.32 μM, while the colorimetric mode shows linear response within 0-200 μM with an instrumental LOD of 18.4 μM and a naked-eye detection limit of 50 μM. This dual-mode sensor tolerates milk-matrix interferences and provides satisfactory recoveries upon simple sample dilution. This work offers a simple dual-mode sensor for rapid food choline detection.
More Related Videos
12:50Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
Published on: June 9, 2014
10:10Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Published on: November 13, 2021
Related Concept Videos
High-Performance Liquid Chromatography: Types of Detectors
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Microbial Biosensors