Steric attenuation of membrane-associated dimerization-induced fluorescence for cell-free biosensing
Related Experiment Video
Updated: Nov 9, 2026

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET
Published on: August 17, 2022
Abstract:
We present a membrane-associated, cell-free biosensing reporter based on rapalog-induced split sfCherry reconstitution. Rapalog increased fluorescence in solution, lipid vesicles, and supported lipid bilayers. Antibodies binding to epitope tags near the dimerization interface reduced the fluorescence signal, consistent with steric interference with reporter assembly.
More Related Videos
10:57Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
Published on: November 2, 2009
14:09Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell
Published on: August 4, 2015