F7 promotes osteoclast precursor migration and osteoclastic bone resorption
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Updated: Nov 9, 2026

Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro
Published on: March 18, 2019
Huaizhi Zhang1, Xu Chen1, Jianhui Dai1
1Department of Minimally Invasive Spinal Surgery, The Affiliated Hospital of Putian University, Putian, Fujian, China.
Objectives:
Blood coagulation factor VII (FVII, F7) acts as a crucial cytokine that promotes cell migration, and its function is exerted through binding to tissue factor (TF). Migration of osteoclast precursors (OCPs) is essential for osteoclast recruitment, fusion and differentiation. Our study aimed to explore the role of F7 in OCP migration and osteoclastogenesis.
Methods:
We first introduced varying concentrations of F7 to observe the migration and differentiation capacities of OCPs. Subsequently, the role of TF in F7-regulated OCP parameters was examined through TF lentiviral silencing. Finally, the effects of F7 on bone parameters, OCP adhesion and osteoclastogenesis in ovariectomized mice were investigated using F7-silenced adeno-associated viruses (AAVs).
Results:
The results showed that F7 promoted OCP migration and osteoclastogenesis in a concentration-dependent manner. The enhancing effects of F7 on OCP migration and osteoclastogenesis were blocked by TF silencing. Furthermore, F7 silencing ameliorated bone loss in ovariectomized mice, accompanied by reduced OCP adhesion and impaired osteoclastogenesis within trabecular bone tissue.
Conclusions:
Collectively, F7 enhances osteoclastic bone resorption by promoting OCP migration.
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