Early-life human lymphopoiesis generates a distinct B-cell lineage
Related Experiment Video
Updated: Nov 9, 2026

Flow Cytometric Characterization of Murine B Cell Development
Published on: January 22, 2021
Abstract:
We characterize the first wave of human B-lymphopoiesis across gestation (n=42 donors) and identify a developmentally and functionally distinct B-cell lineage that shapes antibody specificity before birth. The earliest lymphoid progenitors engage an evolutionarily conserved genetic program enriched for imprinted genes associated with embryogenesis in mice and humans. These progenitors arise in the yolk sac and acquire Early B-cell Factor 1 (EBF1) in the embryonic liver, initiating B-lymphopoiesis before Hematopoietic Stem Cells emerge. Functionally, these early-life B-lymphoid progenitors (eBLPs; CD81 hi TdT - IL3R + ) respond to IL-3 to promote B-cell fate commitment and generate two waves of stereotyped public B-cell receptor (BCR) repertoires, shaping adaptive immunity at birth. The same molecular program and public BCRs are recapitulated during B-cell differentiation from pluripotent stem cells in vitro and after transplantation into humanized mice, where they generate antibody-secreting cells and SOX4 + B cells resembling infant lymphocytes. Developmental origin therefore intrinsically programs B-cell identity and antibody specificity.
More Related Videos
Related Concept Videos
Cells of the Adaptive Immune Response
Primary Lymphoid Organs
The red bone marrow is a soft, spongy tissue nestled in the interior of long bones such as the humerus and femur. It is the site...
Lineage Commitment
B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Production of Formed Elements
Most HSCs commit to...
Special Features of Adaptive Immunity
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...

