Echovirus E6 and E30 serve as key recombination hubs in echovirus evolution
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Updated: Nov 10, 2026

Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection
Published on: May 24, 2020
Manxi Shen1, Guoqing Chen2, Lei Cui3
1Kangda College of Nanjing Medical University Nanjing, Lianyungang, China.
Abstract:
Recombination drives Enterovirus B (EV-B) evolution and pathogenic strain emergence, yet factors governing echovirus (EchoV)-mediated recombination remain undefined. We analyzed 1017 high-quality complete EV-B genomes using Recombination Detection Program 4 and SimPlot for recombination detection, and constructed phylogenetic trees via MEGA7. 31 putative recombination events yielding EchoV recombinant progeny were identified, involving 6 EchoV serotypes. E6, E11, E18, and E30 accounted for 80.6% (25/31) of events: E6 participated in 8 events, E30 in 7 events (including exchanges with Coxsackievirus B4), and E11 and E18 in 5 events each. Recombination hotspots localized to 2 C (45.2%, 14/31), 2 A (22.6%, 7/31), and VP1 (9.7%, 3/31) regions. EchoV E6 and E30 function as core recombination hubs, facilitating cross-serotype and non-EchoV EV-B genomic exchanges. This highlights their role in generating variants linked to hand, foot, and mouth disease and aseptic meningitis.
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