Things We Do for No Reason-Polymerase Chain Reaction for Lyme Disease Diagnostic Testing

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Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR
07:20

Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR

Published on: February 4, 2018

Wesley D Kufel1,2, Alicia A Zimmer2, Rahul Mahapatra2

  • 1Department of Pharmacy Practice, State University of New York at Binghamton School of Pharmacy and Pharmaceutical Sciences, Binghamton, New York, USA.

Open Forum Infectious Diseases
|November 11, 2026
PubMed

Keywords:
Borrelia burgdorferiLyme diseasediagnostic stewardshippolymerase chain reaction

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The polymerase chain reaction, or PCR, is a widely used technique for copying segments of DNA. Due to exponential amplification, PCR can produce millions or billions of DNA copies within just a few hours. In a PCR reaction, a heat-resistant DNA polymerase enzyme amplifies the original DNA through a series of temperature changes inside an automated machine called a thermocycler.PCR is a Versatile Method that Revolutionized Molecular BiologyKary Mullis developed PCR in 1983, for which he was...
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The polymerase chain reaction, or PCR, is a widely used technique for copying segments of DNA. Due to exponential amplification, PCR can produce millions or billions of DNA copies within just a few hours. In a PCR reaction, a heat-resistant DNA polymerase enzyme amplifies the original DNA through a series of temperature changes inside an automated machine called a thermocycler.PCR is a Versatile Method that Revolutionized Molecular BiologyKary Mullis developed PCR in 1983, for which he was...