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Colloidal particle-size determination by gel filtration.

M W Billinghurst, D Jette

    Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
    |February 1, 1979
    PubMed
    Summary

    Gel filtration effectively measures particle sizes below 100 nm for technetium colloids. For larger particles, ultrafiltration offers a more convenient method for size determination.

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    Area of Science:

    • Radiopharmacy
    • Colloid Science
    • Analytical Chemistry

    Background:

    • Technetium-99m (99mTc) radiocolloids are crucial diagnostic agents.
    • Accurate particle size determination is vital for radiopharmaceutical efficacy and safety.
    • Existing methods for particle size analysis have limitations.

    Purpose of the Study:

    • To evaluate gel filtration for particle size determination of 99mTc sulfur colloid and 99mTc antimony sulfide colloid.
    • To compare gel filtration results with electron microscopy and ultrafiltration.
    • To identify the most suitable method for different particle size ranges.

    Main Methods:

    • Particle size analysis using gel filtration chromatography.
    • Comparison with particle size data obtained from electron microscopy.
    • Particle size analysis using ultrafiltration.

    Main Results:

    • Gel filtration accurately determined particle sizes for colloids below 100 nm.
    • Electron microscopy provided complementary size data.
    • Ultrafiltration proved to be the most convenient method for particle sizes exceeding 100 nm.

    Conclusions:

    • Gel filtration is a reliable technique for sub-100 nm radiocolloid particle size analysis.
    • Ultrafiltration is the preferred method for larger radiocolloid particles (>100 nm).
    • Method selection depends on the expected particle size range for optimal radiopharmaceutical characterization.

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