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Replication of mengovirus in HeLa cells preinfected with nonreplicating poliovirus
Abstract:
The replication of mengovirus in HeLa cells preinfected with poliovirus in the presence of 10(-3) M guanidine was investigated. Although host cell protein synthesis is inhibited by the presence of nonreplicating poliovirus, it is found that mengovirus ribonucleic acid (RNA) and protein synthesis proceed normally under the same conditions. Furthermore, no effects on mengovirus growth by poliovirus can be detected either when Mengo protein synthesis is interrupted by Acti-Dione or when its RNA synthesis is reduced by incubation at 28 C. It is suggested that the poliovirus inhibitory factor may be able to distinguish between an RNA element required in the protein-synthesizing apparatus of the host cell and a comparable element in that of the heterologous virus.
Insights
Mengovirus replication occurs normally in HeLa cells despite poliovirus infection and guanidine presence. Poliovirus does not inhibit mengovirus RNA or protein synthesis, suggesting a specific molecular recognition mechanism.
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Background:
- Poliovirus infection typically inhibits host cell protein synthesis.
- Understanding viral interference mechanisms is crucial for antiviral development.
Purpose of the Study:
- To investigate the replication of mengovirus in HeLa cells under conditions of poliovirus coinfection and guanidine treatment.
- To determine if poliovirus inhibits mengovirus RNA and protein synthesis.
Main Methods:
- HeLa cells were preinfected with poliovirus.
- Mengovirus replication was assessed in the presence of 10(-3) M guanidine.
- Mengovirus protein and RNA synthesis were monitored.
- Effects were tested during mengovirus protein synthesis inhibition (Acti-Dione) and RNA synthesis reduction (28 C).
Main Results:
- Mengovirus RNA and protein synthesis proceeded normally despite host cell protein synthesis inhibition by poliovirus.
- Poliovirus did not affect mengovirus growth even when mengovirus protein or RNA synthesis was experimentally hindered.
- The poliovirus inhibitory factor appears to selectively target host cell machinery.
Conclusions:
- Poliovirus does not inhibit mengovirus replication in HeLa cells.
- The poliovirus inhibitory factor likely distinguishes between host and viral RNA elements involved in protein synthesis.
- This suggests a specific molecular interaction rather than a general cellular shutdown.