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Recombination between guanidine-resistant and dextran sulfate-resistant mutants of type 1 poliovirus
Abstract:
Mixed infection of monkey kidney cells with two mutants of the LSc2ab strain of poliovirus, one resistant to guanidine and the other resistant to both dextran sulfate and 2-(alpha-hydroxybenzyl)-benzimidazole (HBB), yielded progeny in which the number of gua(r)dex(r) particles exceeded by a factor of 7 to 10 the expected number of similar particles occurring through spontaneous mutation; recombination would explain the fairly high excess of doubly mutant particles that was obtained. Scoring of HBB resistance in 50 gua(r)dex(r) clones suggested that, during recombination, resistance to dextran sulfate is not associated with HBB resistance.
Insights
Recombination between poliovirus mutants generated more guanidine-resistant, dextran sulfate-resistant particles than expected by chance. This suggests genetic exchange, but dextran sulfate resistance wasn't linked to HBB resistance.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Poliovirus LSc2ab strain mutants were used to study genetic recombination.
- One mutant was resistant to guanidine (gua(r)), the other to dextran sulfate and HBB (dex(r)HBB(r)).
Purpose of the Study:
- To investigate genetic recombination in poliovirus.
- To determine if resistance markers (dextran sulfate and HBB) are linked during recombination.
Main Methods:
- Mixed infection of monkey kidney cells with two distinct poliovirus mutants.
- Quantification of progeny virus particles with combined resistance markers (gua(r)dex(r)).
- Analysis of HBB resistance in guanidine-resistant, dextran sulfate-resistant (gua(r)dex(r)) clones.
Main Results:
- Progeny virus showed a 7-10 fold excess of gua(r)dex(r) particles compared to spontaneous mutation rates.
- Recombination is proposed as the mechanism explaining the high yield of doubly mutant particles.
- Analysis of 50 gua(r)dex(r) clones indicated that dextran sulfate resistance is not associated with HBB resistance during recombination.
Conclusions:
- Genetic recombination occurs in poliovirus LSc2ab strain.
- Dextran sulfate resistance and HBB resistance are independently assorted genetic markers in poliovirus recombination.