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Subcellular distribution of newly synthesized virus-specific polypeptides in Moloney murine leukemia virus infected
Abstract:
Immune precipitation analysis of pulse-labeled proteins present in subcellular fractions of mouse embryo cells infected with Moloney murine leukemia virus showed the presence of anti-gp70 serum-precipitable viral envelope gene products mainly in the microsomal fractions of these cells. In contrast, anti-p30 serum-specific gag (group specific antigen) gene products were found to be distributed in similar amounts in both the microsomal and postmicrosomal supernatant fractions of pulse-labeled cells.
Insights
Moloney murine leukemia virus infection in mouse cells reveals viral envelope proteins in microsomal fractions. Gag gene products, however, distribute evenly between microsomal and supernatant fractions.
Area of Science:
- Virology
- Molecular Biology
- Cellular Biology
Background:
- Moloney murine leukemia virus (MMLV) is a retrovirus that infects mouse cells.
- Understanding the intracellular localization of viral proteins is crucial for comprehending viral replication and assembly.
Purpose of the Study:
- To investigate the subcellular localization of viral envelope (gp70) and gag (p30) gene products in MMLV-infected mouse embryo cells.
Main Methods:
- Immune precipitation analysis was performed on pulse-labeled proteins from subcellular fractions.
- Cells were infected with Moloney murine leukemia virus.
- Specific antibodies against gp70 and p30 were used for precipitation.
Main Results:
- Viral envelope gene products (gp70) were predominantly found in the microsomal fractions.
- Gag gene products (p30) were detected in similar quantities in both microsomal and postmicrosomal supernatant fractions.
Conclusions:
- The subcellular distribution of MMLV viral proteins differs between envelope and gag proteins.
- These findings provide insights into the intracellular trafficking and organization of viral components during MMLV infection.