Related Experiment Videos
Propagation of MM virus in continuous cell lines
Abstract:
Baby hamster kidney (BHK), McCoy, and L cell lines were found to be suitable for isolation of MM virus from infected mouse brain tissue. The virus was recovered in high titer in the first passage in BHK and McCoy cells, with concomitant cytopathic effect (CPE). In L cells, virus yield was lower than in the other two cell lines and CPE was incomplete. Adaptation of the virus to BHK and McCoy cells by serial passages was evidenced by accelerated development of the CPE and increase in the virus titer. Plaques were obtained in all three cell lines when inoculated with infected mouse brain or with the tissue culture-propagated virus. In the BHK cells, the virus release preceded the appearance of CPE and maximal yield of virus was obtained after 1 to 3 days of incubation, depending on the size of inoculum. The BHK-propagated virus had the same lethality for mice as did the mouse brain-propagated stock, and there was no difference in the course of the disease caused by the two preparations.
Insights
Baby hamster kidney (BHK), McCoy, and L cell lines effectively isolate MM virus from mouse brains. BHK and McCoy cells show high virus titers and cytopathic effects (CPE), aiding MM virus adaptation and study.
Area of Science:
- Virology
- Cell Biology
Background:
- MM virus isolation from infected mouse brain tissue is crucial for understanding its pathogenesis.
- Evaluating different cell lines for efficient viral propagation and characterization is essential.
Purpose of the Study:
- To determine the suitability of Baby Hamster Kidney (BHK), McCoy, and L cell lines for MM virus isolation and propagation.
- To characterize the cytopathic effect (CPE) and virus yield in these cell lines.
Main Methods:
- Inoculation of BHK, McCoy, and L cell lines with infected mouse brain tissue.
- Serial passages to adapt the virus and observe changes in CPE and virus titer.
- Plaque assays to quantify infectious virus particles.
Main Results:
- BHK and McCoy cells yielded high titers of MM virus with significant CPE in the first passage.
- L cells showed lower virus yield and incomplete CPE.
- Serial passages in BHK and McCoy cells led to virus adaptation, evidenced by accelerated CPE and increased titers.
- Plaques were successfully obtained in all three cell lines.
Conclusions:
- BHK and McCoy cell lines are highly suitable for MM virus isolation and propagation, facilitating further research.
- The BHK-propagated virus retains its original lethality and disease-causing potential in mice.