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Related Experiment Videos

Enzymatic fluorometry for estimating serum total bile acid concentration.

K Kobayashi, R M Allen, J R Bloomer

    JAMA
    |May 11, 1979
    PubMed
    Summary

    Fasting serum total bile acid (SBA) levels measured by enzymatic fluorometry (EF) effectively distinguish liver disease. EF-SBA assays correlate well with gas-liquid chromatography (GLC) for assessing hepatic function.

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    Area of Science:

    • Biochemistry
    • Hepatology
    • Clinical Chemistry

    Background:

    • Serum total bile acids (SBA) are sensitive indicators of liver function.
    • Accurate and accessible methods for SBA quantification are crucial for diagnosing liver conditions.

    Purpose of the Study:

    • To evaluate the utility of enzymatic fluorometry (EF) for measuring fasting and postprandial serum total bile acids (SBA).
    • To compare EF-SBA levels with liver function status and correlate EF-SBA with gas-liquid chromatography (GLC) methods.

    Main Methods:

    • Serum total bile acid (SBA) levels were measured using enzymatic fluorometry (EF) in subjects with and without liver disease.
    • Fasting and postprandial SBA levels were assessed in normal subjects and cholestatic patients.
    • EF-SBA results were compared with gas-liquid chromatography (GLC) in patients with hepatic lesions.

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    Main Results:

    • SBA levels were significantly elevated in patients with impaired hepatic function compared to healthy controls.
    • Elevated SBA levels were also observed in a subset of patients with hepatic lesions but normal liver function.
    • Enzymatic fluorometry (EF) detected significant postprandial increases in SBA.
    • EF-SBA and GLC-SBA measurements showed a strong correlation (r=0.869).

    Conclusions:

    • Enzymatic fluorometry (EF) is a reliable method for estimating serum total bile acid (SBA) levels.
    • EF-SBA measurements can aid in differentiating liver disease severity and assessing hepatic function.
    • The strong correlation between EF and GLC methods supports the clinical utility of EF-SBA assays.