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Mengovirus-induced cytopathic effect in L-cells: protective effect of interferon
Journal of Virology
|October 1, 1969
Abstract:
The effect of interferon on mengovirus-induced cytopathic effect (CPE) in L cells, the cut-off of host-cell protein synthesis, and production of mature virus were found to be dependent on the concentration of interferon. CPE and inhibition of host protein synthesis were not affected until the concentration of interferon was increased 100-fold over that required to reduce viral yields by 90%.
Insights
Interferon concentration impacts mengovirus infection in L cells. Higher interferon levels are needed to stop host protein synthesis and cytopathic effects than to reduce viral yields.
Area of Science:
- Virology
- Cell Biology
- Immunology
Background:
- Interferons are crucial antiviral proteins.
- Mengovirus infection causes cytopathic effects (CPE) in host cells.
- Host cell protein synthesis is often inhibited during viral infections.
Purpose of the Study:
- To investigate the dose-dependent effects of interferon on mengovirus infection.
- To determine the relationship between interferon concentration and viral yield, CPE, and host protein synthesis inhibition.
Main Methods:
- L cells were infected with mengovirus.
- Varying concentrations of interferon were added to infected cultures.
- Viral yields, CPE, and host cell protein synthesis were measured.
Main Results:
- Interferon's effect on viral yield, CPE, and host protein synthesis was concentration-dependent.
- A 100-fold increase in interferon concentration was required to inhibit CPE and host protein synthesis compared to reducing viral yields by 90%.
Conclusions:
- Interferon exhibits differential dose-dependent effects on various aspects of mengovirus infection.
- The inhibition of viral replication by interferon is more sensitive than the inhibition of cytopathic effects and host protein synthesis.