Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Arbovirus identification by an agar-gel diffusion technique.

C H Calisher, K S Maness

    Applied Microbiology
    |April 1, 1970
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Guidelines to the demarcation of virus species.

    Archives of virology·2017
    Same author

    Emergence and persistence of hantaviruses.

    Current topics in microbiology and immunology·2007
    Same author

    Improved clarity of meaning from the use of both formal species names and common (vernacular) virus names in virological literature.

    Archives of virology·2002
    Same author

    ProMED-mail: background and purpose.

    Emerging infectious diseases·2001
    Same author

    In memoriam Pekka Eljas Halonen (1927-2001).

    Archives of virology·2001
    Same author

    Transmission of an arenavirus in white-throated woodrats (Neotoma albigula), southeastern Colorado, 1995-1999.

    Emerging infectious diseases·2001

    The double diffusion-in-agar test effectively identified precipitation reactions in 75 arboviruses. This method shows promise for the reliable identification of various arboviruses and related viruses.

    Area of Science:

    • Virology
    • Immunology
    • Biochemistry

    Background:

    • Arboviruses represent a significant public health concern, necessitating accurate and efficient identification methods.
    • Existing diagnostic techniques may have limitations in speed or specificity for arbovirus detection.
    • Precipitation reactions in immunological assays offer a potential avenue for viral identification.

    Purpose of the Study:

    • To evaluate the efficacy of the double diffusion-in-agar test for identifying arboviruses.
    • To determine the range of arbovirus groups and related viruses that exhibit specific precipitation reactions using this method.

    Main Methods:

    • A double diffusion-in-agar assay was employed.
    • Seventy-five different arboviruses were tested for precipitation reactions.

    Related Experiment Videos

  • Reactions were analyzed against known viral antigens and antisera.

    • Main Results:

    • Specific precipitation reactions were consistently observed with arboviruses from groups B, California, Simbu, Turlock, and Hart Park.
    • The technique also yielded reactions with vesicular stomatitis virus and other arboviruses.
    • A member of the Tacaribe group and a herpesvirus also showed specific reactions, indicating broader applicability.

    Conclusions:

    • The double diffusion-in-agar test is a feasible and effective method for the identification of arboviruses.
    • This technique can differentiate between various arbovirus groups and may identify related viral families.
    • The study supports the use of this immunological assay in arbovirus diagnostics and research.