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Abstract:
1. The rat kidney kininogenase (KGA) activity was located mainly in the kidney cortex.2. Differential centrifugation of kidney cortex homogenate revealed that the microsomal fraction contained more KGA activity per unit of protein than the other subcellular fractions.3. Subfractionation of the microsomal fraction showed that the KGA activity was recovered in a subfraction also containing high specific activity of both alkaline phosphate and glucose-6-phosphatase. It is suggested that the KGA activity is localized in the plasma membrane and/or endoplasmic reticulum membranes of kidney cortical cells.4. Hydrolysis of N-alpha-benzoyl-L-arginine ethyl ester at pH 8.5 paralleled the KGA activity.