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Characterization of the strain of adenovirus type 7 carrying the defective monkey cell-adapting component
Abstract:
The strain of adenovirus type 7 carrying the defective monkey cell-adapting component (MAC) has been further characterized. MAC is more sensitive to inactivation by ultraviolet light than the associated adenovirus, which, in turn, is more rapidly inactivated than complete simian virus 40 (SV40). The 37% dose was 16 sec for MAC, 60 sec for adenovirus, and 84 sec for SV40. Filtration through membranes revealed that both MAC and adenovirus were retained by 100-mmu filters. MAC equilibrated in cesium chloride at the same buoyant density as the complete adenovirions (1.34 g/ml). Deoxyribonucleic acid extracted from green monkey kidney cells infected with MAC-adenovirus 7 did not hybridize in vitro with SV40 complementary ribonucleic acid. The two components (MAC and adenovirus) of the virus population possess such similar biophysical properties that their separation has not yet been achieved. Evidence to date indicates that MAC is probably not derived from SV40.
Insights
This study characterized a defective monkey cell-adapting component (MAC) in adenovirus type 7. MAC and adenovirus showed distinct UV inactivation rates and similar biophysical properties, suggesting MAC is not SV40-derived.
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Background:
- Adenovirus type 7 can carry defective components.
- Monkey cell-adapting component (MAC) is a defective element associated with adenovirus.
- Understanding MAC's properties is crucial for viral characterization.
Purpose of the Study:
- To further characterize the defective monkey cell-adapting component (MAC) in adenovirus type 7.
- To compare the biophysical properties of MAC with adenovirus and simian virus 40 (SV40).
- To investigate the origin of MAC.
Main Methods:
- Ultraviolet (UV) light inactivation assays.
- Membrane filtration techniques.
- Cesium chloride density gradient centrifugation.
- In vitro nucleic acid hybridization.
Main Results:
- MAC exhibited higher UV sensitivity than adenovirus and SV40.
- Both MAC and adenovirus were retained by 100-mmu filters.
- MAC shared the same buoyant density (1.34 g/ml) as complete adenovirions.
- Extracted DNA from MAC-adenovirus 7 infected cells did not hybridize with SV40 complementary RNA.
- MAC and adenovirus demonstrated similar biophysical properties, hindering separation.
Conclusions:
- MAC is more sensitive to UV inactivation than adenovirus and SV40.
- MAC and adenovirus possess similar biophysical characteristics, making separation difficult.
- Current evidence suggests MAC is not derived from SV40.