Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spin-labeled Neurospora mitochondria.

A Keith, G Bulfield, W Snipes

    Biophysical Journal
    |July 1, 1970
    PubMed
    Summary
    This summary is machine-generated.

    Spin-label studies on Neurospora mitochondria reveal that membrane lipids are fluid. Even when incorporated in vivo, the fatty acid spin labels show motion not significantly restricted by proteins.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Preparation of Entangled States through Hilbert Space Engineering.

    Physical review letters·2016
    Same author

    Genetic control of metabolism: Heritability estimates of enzyme activities in random-bred mice.

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2014
    Same author

    Galton's place among anthropologists.

    The Eugenics review·2011
    Same author

    Misuse of the term evolution.

    Nature·2010
    Same author

    Three Demonstrations ON MALFORMATIONS OF THE HIND END OF THE BODY: Given at the Royal College of Surgeons, England.

    British medical journal·2010
    Same author

    Three Demonstrations ON MALFORMATIONS OF THE HIND END OF THE BODY: Given at the Royal College of Surgeons, England.

    British medical journal·2010
    Same journal

    Tau protein differentially affects Piezo1 and Kir2.1 channels in brain capillary endothelial cells.

    Biophysical journal·2026
    Same journal

    Emergent Intercellular Junction Stability during Cyclic Tissue Loading.

    Biophysical journal·2026
    Same journal

    Enhanced-Sampling Simulations Reveal Distinct Intermediates in SARS-CoV-2 FSE Pseudoknot Interconversion.

    Biophysical journal·2026
    Same journal

    Structure-based simulations of the full Flock House virus capsid reveal pathways and energetics of an infection-critical peptide externalization event.

    Biophysical journal·2026
    Same journal

    Quantifying the Peripheral Surface Information Entropy from Conformational Ensembles of Globular Protein-Peptide Complexes.

    Biophysical journal·2026
    Same journal

    Anisotropic unbinding and location-dependent hovering of a kinesin motor head over microtubule.

    Biophysical journal·2026
    See all related articles

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Background:

    • Understanding mitochondrial membrane dynamics is crucial for cellular respiration and function.
    • Spin-labeling techniques offer insights into the molecular motion and fluidity of biological membranes.

    Purpose of the Study:

    • To investigate the molecular motion of fatty acids within Neurospora mitochondria using spin-labeling.
    • To compare lipid dynamics under in vivo and in vitro labeling conditions.
    • To assess the influence of protein-lipid interactions on membrane fluidity.

    Main Methods:

    • Incorporation of a long-chained spin-labeled fatty acid into Neurospora mitochondria under in vivo and in vitro conditions.
    • Electron paramagnetic resonance (EPR) spectroscopy to analyze the motion of spin labels at various temperatures.

    Related Experiment Videos

  • Comparative analysis with spin-labeled isolated lipids and spin-labeled fatty acid-albumin aggregates.

    • Main Results:

    • Spin-labeled fatty acids were successfully incorporated into mitochondrial phospholipids in Neurospora.
    • Distinct molecular motion patterns were observed for spin labels under in vivo versus in vitro conditions.
    • Comparisons indicated that the hydrocarbon regions of mitochondrial membranes exhibit significant fluidity.

    Conclusions:

    • The fluidity of mitochondrial membranes is largely maintained, with limited restriction of motion by protein associations.
    • In vivo incorporation provides a more physiologically relevant model for studying membrane dynamics.
    • Spin-labeling is a valuable tool for probing membrane fluidity and protein-lipid interactions in biological systems.