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Leukemia viruses associated with mouse myeloma cells
Summary
Murine leukemia virus infects myeloma cells in mice, leading to the secretion of RNA-containing virus particles. These particles share characteristics with other murine leukemia viruses and contain group-specific antigens.
Area of Science:
- Virology
- Immunology
- Oncology
Background:
- Murine myeloma cells are utilized in cancer research.
- Murine leukemia viruses (MLVs) are known oncogenic retroviruses.
Purpose of the Study:
- To investigate the presence and characteristics of murine leukemia virus in myeloma cells.
- To analyze the properties of extracellular and intracellular virus particles.
Main Methods:
- Isolation and characterization of virus particles from myeloma cell lines.
- Density gradient centrifugation to determine virus particle density.
- RNA isolation and base composition analysis.
- Detection of group-specific viral antigens.
Main Results:
- Myeloma cells from BALB/c and C3H mice showed evidence of MLV infection.
- Secreted virus particles had a density of 1.20-1.22 gm/cm³ and contained 74S RNA.
- Intracellular virus particles had a density of 1.29-1.32 gm/cm³.
- Both particle types contained the MLV group-specific antigen.
Conclusions:
- Myeloma cells can harbor and release MLV.
- The isolated virus shares properties with known MLVs.
- The presence of MLV may be relevant to myeloma pathogenesis.