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Related Experiment Videos

Focus formation by a murine sarcoma leukemia virus complex. I. Theoretical analysis.

G M Hahn, A Declève, M Lieberman

    Journal of Virology
    |April 1, 1970
    PubMed
    Summary

    Murine sarcoma-leukemia virus complexes form foci in cell cultures. Quantitative analysis of focus counts allows for the determination of sarcoma virus pseudotype composition and leukemia virus assay.

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    Area of Science:

    • Virology
    • Cell Biology
    • Molecular Biology

    Background:

    • Murine sarcoma-leukemia virus complexes induce cell transformation, forming distinct foci in culture.
    • Previous explanations for titration patterns involved interactions between leukemia, defective sarcoma, and competent hybrid virions.

    Purpose of the Study:

    • To quantitatively analyze the interactions of virions involved in murine sarcoma-leukemia virus complex-induced cell transformation.
    • To derive equations for determining sarcoma virus pseudotype composition and assaying exogenous leukemia virus.

    Main Methods:

    • Quantitative analysis of focus counts versus virus dilutions.
    • Derivation of mathematical equations based on statistical distribution of virions in culture cells.
    • Assumption of equal infection probability for all virions and focus formation upon single-cell transformation.

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    Main Results:

    • Developed a quantitative model for virion interactions in cell transformation.
    • Derived equations enabling the determination of sarcoma virus pseudotype composition.
    • Established a method for the assay of exogenous leukemia virus.

    Conclusions:

    • The quantitative analysis provides a robust method for characterizing virus composition.
    • The derived equations offer a reliable technique for leukemia virus quantification.
    • Discussion of limitations and measurement accuracy associated with the model's assumptions.