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Related Experiment Videos

Host range mutants of polyoma virus.

T L Benjamin

    Proceedings of the National Academy of Sciences of the United States of America
    |September 1, 1970
    PubMed
    Summary

    Researchers developed a polyoma virus mutant that cannot infect normal mouse cells but can infect transformed mouse cells. This mutant is also unable to transform rat or hamster cells, indicating a shared intracellular block for viral replication and cell transformation.

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    Area of Science:

    • Virology
    • Cell Biology
    • Molecular Biology

    Background:

    • Polyoma virus is a DNA tumor virus that can infect rodent cells.
    • Understanding the viral life cycle and transformation mechanisms is crucial for cancer research.

    Purpose of the Study:

    • To isolate and characterize polyoma virus mutants with altered host cell permissivity.
    • To investigate the intracellular steps involved in polyoma virus replication and cell transformation.

    Main Methods:

    • Isolation of a polyoma-transformed mouse cell line susceptible to lytic infection.
    • Selection of polyoma virus mutants based on differential growth in transformed versus untransformed cells.
    • Assessment of viral DNA replication and cell transformation capabilities of the mutants.

    Main Results:

    • A polyoma virus mutant was selected that grows in transformed mouse cells but not in parental mouse cells.
    • This mutant exhibits defects in transforming rat and hamster cells.
    • Viral DNA from mutants shows the same host range as the whole virus, suggesting an intracellular block.

    Conclusions:

    • The identified polyoma virus mutants are blocked at an intracellular stage essential for both viral replication in mouse cells and transformation of rodent cells.
    • This finding provides insights into the molecular mechanisms underlying polyoma virus pathogenesis and oncogenesis.

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