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Concentration of enteroviruses on membrane filters
Abstract:
Enteroviruses can be made to adsorb or to pass through membrane filters by manipulation of the suspending medium. Salts facilitate virus adsorption, but membrane-coating components (MCC) interfere. Because cells release MCC into the culture medium during viral growth, MCC must be removed before virus can be adsorbed to membranes. Adsorbed virus can be eluted with diluents containing MCC (cell extracts or serum) or agents that reduce surface tension (sodium lauryl sulfate). By membrane adsorption and elution, enteroviruses can be readily concentrated and quantitatively recovered from crude virus harvests.
Insights
Manipulating suspending media allows enteroviruses to bind or pass through filters. Salts aid virus adsorption, while membrane-coating components (MCC) hinder it, requiring removal for effective concentration and recovery.
Area of Science:
- Virology
- Biochemistry
Background:
- Enteroviruses are common human pathogens.
- Viral concentration is crucial for detection and study.
Purpose of the Study:
- To investigate methods for concentrating enteroviruses using membrane filtration.
- To identify factors affecting virus adsorption and elution from membranes.
Main Methods:
- Membrane filtration was used to adsorb and elute enteroviruses.
- The effects of salts and membrane-coating components (MCC) on adsorption were examined.
- Elution was performed using MCC-containing diluents or surface tension-reducing agents.
Main Results:
- Salts facilitate enterovirus adsorption to membranes.
- Membrane-coating components (MCC) released by cells interfere with adsorption.
- MCC or sodium lauryl sulfate effectively elute adsorbed enteroviruses.
- This method allows for quantitative recovery of enteroviruses from crude harvests.
Conclusions:
- Membrane adsorption and elution is an effective technique for concentrating enteroviruses.
- Understanding the role of MCC and salts is key to optimizing viral recovery.
- This method facilitates the concentration and quantitative recovery of enteroviruses.