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Effect of cellulose sulphate on serum complement.

V Eisen, C Loveday

    British Journal of Pharmacology
    |August 1, 1970
    PubMed
    Summary

    Cellulose sulfate reduces complement levels in human serum, unlike its clot-promoting effects. This reduction is independent of clotting factor XII, offering new insights into its biological actions.

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    Area of Science:

    • Biochemistry
    • Immunology
    • Hematology

    Background:

    • Cellulose sulfate exhibits procoagulant and plasma kinin-generating properties.
    • Its interaction with the complement system in human serum was previously uncharacterized.

    Purpose of the Study:

    • To investigate the effect of cellulose sulfate on complement activity in human serum.
    • To determine if the complement-reducing effect is mediated by clotting factor XII.

    Main Methods:

    • Human serum was treated with varying concentrations of cellulose sulfate (50-600 µg/ml).
    • Complement titres were measured using standard assays.
    • The role of clotting factor XII was assessed by comparing results in factor XII-deficient serum or by specific inhibition.

    Main Results:

    • Cellulose sulfate significantly reduced complement titres in human serum across the tested concentration range.
    • This inhibitory effect on complement was observed independently of the known procoagulant and plasma kinin-forming actions of cellulose sulfate.
    • The reduction in complement titres was not mediated by clotting factor XII.

    Conclusions:

    • Cellulose sulfate possesses a distinct inhibitory effect on the human complement system.
    • This action is separate from its effects on blood coagulation and kinin release.
    • The findings suggest a novel biological activity of cellulose sulfate, not involving clotting factor XII.

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